Application
Western blot analysis of PI 3-kinase p85β expression in non-transfected: (A) and human PI 3-kinase p85β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PI 3-kinase p85α expression in non-transfected: (A) and mouse PI 3-kinase p85α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PI 3-kinase p85α expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of PI 3-kinase p85β expression in non-transfected: (A) and mouse PI 3-kinase p85β transfected: (B) 293T whole cell lysates.| Product Name | PI 3-kinase p85α Antibody (Z-8) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 333-430 of the 85 kDa subunit of PI 3-kinase of human origin |
| Modification Notes | Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85å and p85∫), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110å and p110∫ interact with p85å, and p110å has also been shown to interact with p85∫ in vitro. p110∂ expression is restricted to white blood cells. It has been shown to bind p85å and ∫, but it apparently does not phosphorylate these subunits. p110∂ seems to have the capacity to autophosphorylate. p110© does not interact with the p85 subunits. It has been shown to be activated by å and ∫© heterotrimeric G proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PI 3-kinase p85α, p85β and p55 PIK of moue, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 333-430 of the 85 kDa subunit of PI 3-kinase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 333-430 of the 85 kDa subunit of PI 3-kinase of human origin |
| Entrez Gene | 5295 |
|---|
Application
Western blot analysis of PI 3-kinase p85β expression in non-transfected: (A) and human PI 3-kinase p85β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PI 3-kinase p85α expression in non-transfected: (A) and mouse PI 3-kinase p85α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PI 3-kinase p85α expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of PI 3-kinase p85β expression in non-transfected: (A) and mouse PI 3-kinase p85β transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PI 3-kinase p85α, p85β and p55 PIK of moue, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
