Application
Western blot analysis of PhLP expression in non-transfected: (A) and human PhLP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PhLP expression in non-transfected 293T: (A), human PhLP transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | PhLP Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 66-175 mapping within an internal region of PhLP of human origin |
| Modification Notes | Phosducin-like protein (PhLP, PDCL) is an ethanol-responsive modulator of heterotrimeric G proteins (1). The protein shares extensive amino acid sequence homology with phosducin (Phd), a phosphoprotein expressed in retina and pineal gland. Both PhLP and Phd regulate G-protein signaling by binding to the beta-gamma subunits of G proteins (2,3). PhLP interacts with G beta-gamma via a short C-terminal binding site (4). Additionally, PhLP acts as a substrate for GRK2 phosphorylation at the same C-terminal binding site between residues 195 and 218 (5). PhLPs may participate directly in the regulation of calcium-evoked exocytosis in adrenal medullary chromaffin cells (6). Glycosylated PhLP regulates opioid receptor function in mouse brain (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PhLP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 66-175 mapping within an internal region of PhLP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 66-175 mapping within an internal region of PhLP of human origin |
| Entrez Gene | 5082 |
|---|
Application
Western blot analysis of PhLP expression in non-transfected: (A) and human PhLP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PhLP expression in non-transfected 293T: (A), human PhLP transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of PhLP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
