Application
Western blot analysis of BLM hydrolase expression in non-transfected: (A) and mouse BLM hydrolase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BLM hydrolase expression in non-transfected: (A) and mouse BLM hydrolase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BLM hydrolase expression in non-transfected: (A) and mouse BLM hydrolase transfected: (B) 293T whole cell lysates.| Product Name | BLM hydrolase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of BLM hydrolase of human origin |
| Modification Notes | BLM hydrolase (Bleomycin hydrolase, BLM hydrolase, BMH) is a 455 amino acid protein encoded by the human gene BLMH. BLM hydrolase belongs to the cysteine protease papain superfamily and the peptidase C1 family. It is a cytoplasmic cysteine peptidase commonly found as a homohexamer. It is highly conserved through evolution, however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM). BLM is an essential component of combination chemotherapy regimens for cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BLM hydrolase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of BLM hydrolase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of BLM hydrolase of human origin |
| Entrez Gene | 642 |
|---|
Application
Western blot analysis of BLM hydrolase expression in non-transfected: (A) and mouse BLM hydrolase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BLM hydrolase expression in non-transfected: (A) and mouse BLM hydrolase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BLM hydrolase expression in non-transfected: (A) and mouse BLM hydrolase transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of BLM hydrolase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
