Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of CBX7 expression in non-transfected: (A) and mouse CBX7 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CBX7 expression in SK-N-MC nuclear extract.| Product Name | CBX7 Antibody (P-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | CBX7 (Chromobox protein homolog 7) is a 251 amino acid nuclear protein that contains one N-terminal chromo domain and one C-terminal Pc box. Highly expressed in kidney, brain, heart and skeletal muscle, with weaker expression in peripheral blood leukocytes, CBX7 functions as a component of the chromatin-associated polycomb complex (PcG) and is involved in maintaining the transcriptionally repressed state of target genes. Additionally, CBX7 modifies chromatin and is thought to extend the cellular life span of epithelial cells by repressing p14 ARF expression, while simultaneously repressing telomerase activity. Due to its ability to repress the transcription of cell-cycle related proteins, CBX7 is thought to play a role in tumorigenesis, specifically in the development of follicular lymphoma and thyroid cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CBX7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of CBX7 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of CBX7 of human origin |
| Entrez Gene | 23492 |
|---|
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of CBX7 expression in non-transfected: (A) and mouse CBX7 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CBX7 expression in SK-N-MC nuclear extract.| Application Notes | recommended for detection of CBX7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
