Application
Western blot analysis of p-E2F-1 expression in T47D (A), Hep G2 (B), and A2058 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.| Product Name | p-E2F-1 Antibody (Ser 337) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex. Human E2F-1 may be phosphorylated at Ser 337 during the S phase of the cell cycle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 337 phosphorylated E2F-1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 337 phosphorylated E2F-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 1869 |
|---|
Application
Western blot analysis of p-E2F-1 expression in T47D (A), Hep G2 (B), and A2058 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
