Application
Western blot analysis of MARCH8 expression in K-562 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic staining.| Product Name | MARCH8 Antibody (I-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. MARCH8 (membrane-associated ring finger (C3HC4) 8), also known as MIR or RNF178 (RING finger protein 178), is a 291 amino acid multi-pass membrane protein that localizes to vesicle membranes and contains one RING-CH-type zinc finger. Expressed in a variety of tissues, including immature dendritic cells, MARCH8 functions as an E3 ubiquitin ligase that is thought to regulate immune responses by promoting the ubiquitination and subsequent degradation of target proteins, such as B7-2 and CD71. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MARCH8 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of MARCH8 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 220972 |
|---|
Application
Western blot analysis of MARCH8 expression in K-562 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic staining.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
