Application
Western blot analysis of MAGE-A2 expression in HL60 whole cell lysate.| Product Name | MAGE-A2B Antibody (N-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of MAGE-A2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The melanoma-associated antigen (MAGE) family consists of a number of antigens recognized by cytotoxic T lymphocytes. The MAGE genes were initially isolated from different kinds of tumors and, based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and in normal testis and placenta. MAGE-A2 (melanoma antigen family A2), also known as MAGE2, MAGEA2A, MAGE-A2B or CT1.2 (cancer/testis antigen 1.2), is a 314 amino acid protein that contains one MAGE domain. Expressed in a variety of carcinomas, including melanoma, breast cancer, lung cancer and neck and head squamous cell carcinoma, MAGE-A2/2B is thought to play a role in embryonic development and tumor transformation/progression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MAGE-A2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of MAGE-A2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 4101 |
|---|
Application
Western blot analysis of MAGE-A2 expression in HL60 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
