Application
Western blot analysis of PRAT4A expression in 293T whole cell lysate.Application
Western blot analysis of PRAT4A expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human muscle tissue showing cytoplasmic staining.| Product Name | PRAT4A Antibody (E-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Toll-like receptors (TLRs) are responsible for the innate recognition of microbial products and the induction of immune responses. There are two types of TLRs, cell surface and intracellular, but both rely on their subcellular distribution for optimal microbial recognition. TLR4, a cell surface TLR, is a member of the Toll family that detects lipopolysaccharide (LPS), which is an endotoxin that activates immune cells such as macrophages and dendritic cells. LPS recognition by TLR4 is induced by MD-2, an extracellular molecule that binds to the extracellular domain of TLR4, and PRAT4A (Protein Associated with Toll-Like Receptor 4), which regulates the cell surface expression of TLR4. PRAT4A, which is also known as CAG4A or TNRC5, is a 278 amino acid protein and is expressed as two isoforms due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PRAT4A of human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against PRAT4A of human origin |
|---|---|
| Tissue Specificity | raised against PRAT4A of human origin |
| Entrez Gene | 10695 |
|---|
Application
Western blot analysis of PRAT4A expression in 293T whole cell lysate.
Application
Western blot analysis of PRAT4A expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human muscle tissue showing cytoplasmic staining.| Application Notes | recommended for detection of PRAT4A of human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
