Application
Western blot analysis of Wee 1 phosphorylation in non-transfected: (A) and mouse Wee 1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | p-Wee 1 Antibody (Ser 53) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Phosphorylation of Cdc2 on threonine 14 and tyrosine 15 is required to maintain Cdc2 in an inactive state throughout the S and G2 phases of the cell cycle. The human Wee 1 gene encodes a tyrosine-specific protein kinase, designated Wee 1, that phosphorylates Cdc2 on tyrosine 15. Myt 1, a member of the Wee 1 family of protein kinases, has been shown to phosphorylate Cdc2 on both threonine 14 and tyrosine 15 in a cyclin-dependent manner. Activity of both Wee 1 and Myt 1 is regulated during the cell cycle, suggesting that both proteins play a role in mitotic control. Dephosphorylation of Cdc2 on threonine 14 and tyrosine 15 in late G2 by Cdc25 then activates the Cdc2/cyclin B complex to allow entry into mitosis. Human Wee 1 is subject to phosphorylation during the M and G1 phases of the cell cycle and may be phosphorylated on a variety of amino acid residues, including Ser 53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 53 phosphorylated Wee 1 of human origin and correspondingly Ser 52 phosphorylated Wee 1 of mouse and rat origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 53 phosphorylated Wee 1 of human origin and correspondingly Ser 52 phosphorylated Wee 1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 7465 |
|---|
Application
Western blot analysis of Wee 1 phosphorylation in non-transfected: (A) and mouse Wee 1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
