Application
Western blot analysis of UBE2D3 expression in non-transfected: (A) and human UBE2D3 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of UBE2D1 expression in non-transfected 293T: (A), mouse UBE2D1 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of UBE2D1 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | UBE2D1 Antibody (C-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | UBE2D1 (ubiquitin-conjugating enzyme E2D1 or UBC5A), UBE2D2 (ubiquitin-conjugating enzyme E2D2 or UBC5B), and UBE2D3 (ubiquitin-conjugating enzyme E2D3 or UBC5C) are E2 ubiquitin-conjugating enzymes, components of the protein ubiquitination pathway. Protein ubiquitination covalent modification targets proteins for 26 S proteasome-dependent degradation. 3 classes of enzymes influence the conjugation mechanism of ubiquitin to protein. E1 ubiquitin-activating enzymes mediate ATP-dependent charging of ubiquitin via formation of a high energy thiol ester bond between the C-terminus of ubiquitin and a cysteine within itself. Thiol ester-linked ubiquitin is then transferred from E1 to a cysteine residue in an E2 ubiquitin-conjugating enzyme. E2 enzymes in conjunction with E3 ubiquitin-protein ligases transfer ubiquitin monomers and polyubiquitin chains to the substrate target protein, where stable isopeptide linkages are formed. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of UBE2D1 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of UBE2D1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 7321 |
|---|
Application
Western blot analysis of UBE2D3 expression in non-transfected: (A) and human UBE2D3 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of UBE2D1 expression in non-transfected 293T: (A), mouse UBE2D1 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of UBE2D1 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
