Application
p-SH-PTP2 (Tyr 542): : Western blot analysis of phosphorylated SH-PTP2 expression in untreated (A) and insulin-treated (B) NIH/3T3 whole cell lysates.Application
p-SH-PTP2 (Tyr 542): : Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Product Name | p-SH-PTP2 Antibody (Tyr 542) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 542 phosphorylated SH-PTP2 of mouse, rat and human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 542 of SH-PTP2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 542 of SH-PTP2 of human origin |
| Entrez Gene | 5781 |
|---|
Application
p-SH-PTP2 (Tyr 542): : Western blot analysis of phosphorylated SH-PTP2 expression in untreated (A) and insulin-treated (B) NIH/3T3 whole cell lysates.
Application
p-SH-PTP2 (Tyr 542): : Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Application Notes | recommended for detection of Tyr 542 phosphorylated SH-PTP2 of mouse, rat and human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
