Application
Western blot analysis of CstF-77 expression in non-transfected 293T: (A), human CstF-77 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CstF-77 expression in HL-60 whole cell lysate.Application
Western blot analysis of CstF-77 expression in non-transfected 293T: (A), human CstF-77 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CstF-77 expression in Jurkat whole cell lysate.| Product Name | CstF-77 Antibody (K-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against CstF-77 of human origin |
| Modification Notes | Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an å-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of CstF-77 of mouse, rat, human, canine and zebrafish origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against CstF-77 of human origin |
|---|---|
| Tissue Specificity | raised against CstF-77 of human origin |
| Entrez Gene | 1479 |
|---|
Application
Western blot analysis of CstF-77 expression in non-transfected 293T: (A), human CstF-77 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CstF-77 expression in HL-60 whole cell lysate.
Application
Western blot analysis of CstF-77 expression in non-transfected 293T: (A), human CstF-77 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CstF-77 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of CstF-77 of mouse, rat, human, canine and zebrafish origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
