Application
Antibodies tested include p-Lck (Tyr 393): (A,B) and Lck (H-95): (C,D).Application
Antibodies tested include p-Lck (Tyr 393): (A,B,C) and Lck (H-95): (D,E,F).Application
Western blot analysis of Lck phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase treated (C) Jurkat whole cell lysates.Application
Western blot analysis of Lck phosphorylation in untreated (A) and lambda protein phosphatase treated (B) CCRF-HSB-2 whole cell lysates.Application
Blots were probed with p-Lck (Tyr 394): preincubated with its cognate phosphorylated peptide (A) and p-Lck (Tyr 394): (B).| Product Name | p-Lck Antibody (Tyr 394) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 394 of Lck of human origin |
| Modification | Phosphoralated |
| Modification Notes | The Src gene family of membrane-associated protein tyrosine kinases include c-Src, c-Yes, Fyn, Lck, Hck, Lyn, Blk and c-Fgr (1). The human Lck gene encodes a lymphocyte-specific tyrosine kinase designated pp56 Lck. Rearrangement and overexpression of the Lck gene occurs in select murine lymphomas (2,3). Human lymphomas and neuroblastomas frequently exhibit chromosomal abnormalities within a site in the genome that contains the Lck gene (4). Resting T cells contain high levels of the Lck protein and mRNA, both of which decline upon activation of T cells (5,6). Lck expression may contribute to the pathogenesis of some types of neoplastic disease (5,6). Csk phosphorylates Lck on Tyr 505 (7,9). This phosphorylation event supresses Lck catalytic activity under certain conditions (7,8). The phosphorylation of Lck at Tyr 394 stimulates Lck activity regardless of Tyr 505 phosphorylation status (8, 9). In response to T cell antigen receptor binding and subsequent signaling, Lck undergoes phosphorylation on residues that include Tyr-192, Ser-59 and Ser-194. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Tyr 394 phosphorylated Lck of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 394 of Lck of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 394 of Lck of human origin |
| Entrez Gene | 3932 |
|---|
Application
Antibodies tested include p-Lck (Tyr 393): (A,B) and Lck (H-95): (C,D).
Application
Antibodies tested include p-Lck (Tyr 393): (A,B,C) and Lck (H-95): (D,E,F).
Application
Western blot analysis of Lck phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
Application
Western blot analysis of Lck phosphorylation in untreated (A) and lambda protein phosphatase treated (B) CCRF-HSB-2 whole cell lysates.
Application
Blots were probed with p-Lck (Tyr 394): preincubated with its cognate phosphorylated peptide (A) and p-Lck (Tyr 394): (B).| Application Notes | recommended for detection of Tyr 394 phosphorylated Lck of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
