Application
Western blot analysis of phosphorylated Gab 1 expression in untreated (A) and EGF-treated (B) HUVEC whole cell lysates.| Product Name | p-Gab 1 Antibody (Tyr 627) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 627 of Gab 1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The insulin receptor substrate (IRS) family of proteins mediate a variety of intracellular signaling pathways by serving as signaling platforms downstream of several receptor tyrosine kinases, including the insulin and insulin-like growth factor (IGF-1) receptors. Gab 1 (Grb2-associated binder, one such member of the IRS family, plays an important role in cellular growth response, transformation and apoptosis . Gab 1 is a multi-substrate docking protein that functions downstream in the signaling pathways of different receptor kinases, including EGFR. Gab1 is tyrosine phosphorylated normally in response to insulin and consequently, enhances phosphatidylinositol 3-kinase (PI3K) binding. In response to osmotic shock, tyrosine-phosphorylated Gab 1 (p-Gab 1) also binds and activates phosphatidylinositol 3-kinase (PI3K), suggesting that Gab 1 is the major site for PI3K recruitment following osmotic shock stimulation. In the Flt3 ligand-responsive cells, Gab 1 is also rapidly tyrosine phosphorylated after receptor tyrosine kinase Flt3 ligand simulation and interacts with tyrosine-phosphorylated Shp-2, p85, Grb2 and Shc proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Tyr 627 phosphorylated Gab 1 of human and rat origin and correspondingly phosphorylated Tyr 628 of mouse origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 627 of Gab 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 627 of Gab 1 of human origin |
| Entrez Gene | 2549 |
|---|
Application
Western blot analysis of phosphorylated Gab 1 expression in untreated (A) and EGF-treated (B) HUVEC whole cell lysates.| Application Notes | recommended for detection of Tyr 627 phosphorylated Gab 1 of human and rat origin and correspondingly phosphorylated Tyr 628 of mouse origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
