Application
Western blot analysis of ERα expression in MCF7 (A) and ZR-75-1 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed T-47D cells showing nuclear localization.Application
Western blot analysis of human recombinant ERα.| Product Name | ERα Antibody (G-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the hinge region of estrogen receptor of ERα of human origin |
| Modification Notes | Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERå and ER∫, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus , though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERå and ER∫ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERå and ER∫ may be regulated by distinct mechanisms even though they share many functional characteristics. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of estrogen receptor α of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the hinge region of estrogen receptor of ER&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the hinge region of estrogen receptor of ERα of human origin |
| Entrez Gene | 2099 |
|---|
Application
Western blot analysis of ERα expression in MCF7 (A) and ZR-75-1 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed T-47D cells showing nuclear localization.
Application
Western blot analysis of human recombinant ERα.| Application Notes | recommended for detection of estrogen receptor α of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
