Application
Western blot analysis of Squalene epoxidase expression in 721B whole cell lysate.| Product Name | Squalene epoxidase Antibody (L-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Several proteins mediate the biosynthesis of cholesterol. The first specific step in the cholesterol biosynthetic pathway is the conversion of transfarnesyl-diphosphate to Squalene, which is catalyzed by the endoplasmic reticulum membrane-associated enzyme Squalene synthetase, also designated Squalene synthase and Farnesyl-diphosphate farnesyltransferase. Squalene synthetase is located at a branch point in the mevalonate pathway and is also involved in isoprenoid biosynthesis. Squalene epoxidase, also designated Squalene monooxygenase, is a multi-pass microsomal membrane-associated enzyme that catalyzes the first oxygenation step in sterol biosynthesis and most likely functions as one of the rate-limiting enzymes in this pathway. Squalene epoxidase may form a complex with Squalene synthetase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Squalene epoxidase of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Squalene epoxidase of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 6713 |
|---|
Application
Western blot analysis of Squalene epoxidase expression in 721B whole cell lysate.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
