Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and pancreatic duct cells.Application
Western blot analysis of GPR30 expression in SK-BR-3 (A) and MCF7 (B) whole cell lysates.| Product Name | GPR30 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates, and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. G protein receptor 30 (GPR30), also designated chemokine receptor-like 2 (CMKRL2), is a 375-amino acid protein orphan GCPR. GRP30 is an intracellular transmembrane estrogen receptor localized to the endoplasmic reticulum which binds estrogen and estrogen derivatives. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GPR30 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 76-375 mapping at the C-terminus of GPR30 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 76-375 mapping at the C-terminus of GPR30 of human origin |
| Entrez Gene | 2852 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and pancreatic duct cells.
Application
Western blot analysis of GPR30 expression in SK-BR-3 (A) and MCF7 (B) whole cell lysates.| Application Notes | recommended for detection of GPR30 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
