Application
Western blot analysis of ARD expression in non-transfected: (A) and human ARD transfected: (B) 293T whole cell lysates.| Product Name | ARD Antibody (H-138) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 34-171 mapping within an internal region of ARD of human origin |
| Modification Notes | ARD (acireductone dioxygenase), also known as ADI1, APL1, SIPL, SIP-L or MTCBP1, is a 179 amino acid protein that localizes to the nucleus, as well as to the cytoplasmic side of the cell membrane, and belongs to the aci-reductone dioxygenase family of metal-binding enzymes. Expressed in brain, heart, lung, colon, liver, kidney, spleen and skeletal muscle, ARD uses nickel as a cofactor to catalyze a crucial step in the L-methionine biosynthetic pathway, namely the creation of L-methionine from (S)-methyl-5-thio-alpha-D-ribose 1-phosphate. Additionally, ARD interacts with MT-MMP-1 and may be able to down-regulate MT-MMP-1-mediated cell migration. Multiple isoforms of ARD exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ARD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 34-171 mapping within an internal region of ARD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 34-171 mapping within an internal region of ARD of human origin |
| Entrez Gene | 55256 |
|---|
Application
Western blot analysis of ARD expression in non-transfected: (A) and human ARD transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ARD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
