Application
Western blot analysis of PKAα/β/γ cat expression in NIH/3T3 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of truncated human recombinant PKAα/β/&gamama; cat fusion protein.| Product Name | PKAα/β/γ cat Antibody (H-56) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 231-286 mapping near the C-terminus of PKAα catalytic subunit of human origin |
| Modification Notes | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of cAMP-dependent protein kinases (cAPKs or PKAs). Activation of PKAs occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Three catalytic subunits of the PKA holoenzyme exist and are designated PKAå cat, PKA∫ cat and PKA© cat, each of which represent specific gene products. PKAå cat and PKA∫ cat are closely related (93% amino acid sequence similarity), whereas PKA© cat displays 83% and 79% similarity to PKAå cat and PKA∫ cat, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus, where it phosphorylates CREB, leading to TFIIB-TBP1 binding and, ultimately, the linking of phospho-CREB to the Pol II transcription initiation complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PKAα/β cat of mouse, rat and human origin and PKAγ cat of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-286 mapping near the C-terminus of PKA&alpha catalytic subunit of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-286 mapping near the C-terminus of PKAα catalytic subunit of human origin |
Application
Western blot analysis of PKAα/β/γ cat expression in NIH/3T3 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of truncated human recombinant PKAα/β/&gamama; cat fusion protein.| Application Notes | recommended for detection of PKAα/β cat of mouse, rat and human origin and PKAγ cat of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
