Application
Western blot analysis of ATF-2 phosphorylation in human melanoma cells treated with varying doses of the radiomimetic drug neocarzinostatin (NCS).| Product Name | p-ATF-2 Antibody (Ser 490/Ser 498) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 490 and Ser 498 dually phosphorylated ATF-2 of human origin and correspondingly Ser 472 and Ser 480 dually phosphorylated ATF-2 of rat origin of h and r origin by WB, IP and IF |
| Modification | Phosphoralated |
| Modification Notes | Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1. Phosphorylation of human and rat ATF-2 on serine residues 490 and 498 causes translocation to DNA repair foci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 490 and Ser 498 dually phosphorylated ATF-2 of human origin and correspondingly Ser 472 and Ser 480 dually phosphorylated ATF-2 of rat origin of h and r origin by WB IP and IF |
|---|---|
| Tissue Specificity | recommended for detection of Ser 490 and Ser 498 dually phosphorylated ATF-2 of human origin and correspondingly Ser 472 and Ser 480 dually phosphorylated ATF-2 of rat origin of h and r origin by WB, IP and IF |
| Entrez Gene | 1386 |
|---|
Application
Western blot analysis of ATF-2 phosphorylation in human melanoma cells treated with varying doses of the radiomimetic drug neocarzinostatin (NCS).| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
