Application
Western blot analysis of c-Kit phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) A-431 whole cell lysates.Application
Western blot analysis of phosphorylated c-Kit expression in SCF-treated 293 transfected whole cell lysate.| Product Name | p-c-Kit Antibody (Tyr 721) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 721 of c-Kit of human origin |
| Modification | Phosphoralated |
| Modification Notes | The c-Kit proto-oncogene has been identified as a member of the receptor tyrosine kinase family and more specifically has been shown to be closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene, v-Kit, encodes a transmembrane receptor. c-Kit has also been show to be identical with the product of the W locus in mice and as such is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and shown to be encoded at the murine steel (SI) locus. Two sites on c-Kit are able to bind SH2(CHK), the Tyr 568/570 diphosphorylated sequence and the monophosphorylated Tyr 721 sequence. SH2(CHK) binds to the two sites directly and only the Tyr 568/570, and not the Tyr 721, is able to bind SH2(CHK). The Tyr 568 and Tyr 570 residues are phosphorylated in vivo following ligand-stimulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Tyr 721 phosphorylated c-Kit of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 721 of c-Kit of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 721 of c-Kit of human origin |
| Entrez Gene | 3815 |
|---|
Application
Western blot analysis of c-Kit phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) A-431 whole cell lysates.
Application
Western blot analysis of phosphorylated c-Kit expression in SCF-treated 293 transfected whole cell lysate.| Application Notes | recommended for detection of Tyr 721 phosphorylated c-Kit of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
