Application
Western blot analysis of PSMD2 expression in SK-N-SH whole cell lysate.Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).| Product Name | PSMD2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S Proteasome. The 26S Proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains. It is made up of two multisubunit complexes: the 20S Proteasome chamber, which serves as the proteolytic core of the complex and two 19S regulatory particles which recognize and unfold ubiquitinated proteins. PSMD2 (proteasome (prosome, macropain) 26S subunit, non-ATPase 2), also known as S2, TRAP2 (tumor necrosis factor type 1 receptor-associated protein 2) or p97, is a regulatory component of the 26S proteasome. It is expressed in skeletal muscle, brain, liver, placenta, kidney, pancreas, lung and heart. PSMD2 is one of the non-ATPase regulatory subunits of the 19S regulator lid and is implicated in substrate recognition and binding. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PSMD2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 491-790 mapping near the C-terminus of PSMD2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 491-790 mapping near the C-terminus of PSMD2 of human origin |
| Entrez Gene | 5708 |
|---|
Application
Western blot analysis of PSMD2 expression in SK-N-SH whole cell lysate.
Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PSMD2 expression in non-transfected: (A) and mouse PSMD2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).| Application Notes | recommended for detection of PSMD2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
