Application
Western blot analysis of CHIP expression in non-transfected 293T: (A), human CHIP transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of CHIP expression in non-transfected 293T: (A), mouse CHIP transfected 293T: (B) and HEK293 (C) whole cell lysates.Application
Western blot analysis of CHIP expression in non-transfected 293T: (A), human CHIP transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | CHIP Antibody (H-231) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 73-303 mapping at the C-terminus of CHIP of human origin |
| Modification Notes | CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CHIP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 73-303 mapping at the C-terminus of CHIP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 73-303 mapping at the C-terminus of CHIP of human origin |
| Entrez Gene | 10273 |
|---|
Application
Western blot analysis of CHIP expression in non-transfected 293T: (A), human CHIP transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of CHIP expression in non-transfected 293T: (A), mouse CHIP transfected 293T: (B) and HEK293 (C) whole cell lysates.
Application
Western blot analysis of CHIP expression in non-transfected 293T: (A), human CHIP transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of CHIP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
