Application
Western blot analysis of AKAP 149 expression in non-transfected: (A) and mouse AKAP 149 transfected: (B) 293T whole cell lysates.| Product Name | AKAP 149/121 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. This family of proteins has been designated A-kinase anchoring proteins (AKAP). Members of this family, including MAP2 (microtubule-associated protein 2), neuronally expressed AKAP 79 and AKAP 150, and the DNA binding AKAP 95, display differential tissue specificity and localization. AKAP 149, the human homolog of mouse and rat AKAP 121, is a splice variant of S-AKAP 84 and may be involved in the phosphorylation-dependent regulation of RNA processing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AKAP 149 of human origin and AKAP 121 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 604-903 mapping at the C-terminus of AKAP 149 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 604-903 mapping at the C-terminus of AKAP 149 of human origin |
| Entrez Gene | 8165 |
|---|
Application
Western blot analysis of AKAP 149 expression in non-transfected: (A) and mouse AKAP 149 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of AKAP 149 of human origin and AKAP 121 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
