Application
Western blot analysis of ERI-1 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of ERI-1 expression in non-transfected: (A) and human ERI-1 transfected: (B) 293T whole cell lysates.| Product Name | ERI-1 Antibody (H-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 161-295 mapping within an internal region of ERI-1 of human origin |
| Modification Notes | Helicase with RNase motif, more commonly designated Dicer, cleaves double-stranded RNA (dsRNA) in the RNA interference and small temporal RNA (stRNA) pathways, producing active small RNA components (siRNAs) which target the destruction of RNA and repress gene expression. Human Dicer cleaves dsRNA independent of ATP. The 3’-5’ exonuclease ERI-1, also known as Protein 3'hExo, degrades Histone mRNA after replication and may be involved in the regulation of RNA interference. ERI-1 has a high affinity for the stem-loop structure of replication-dependent Histone pre-mRNAs. It requires the 5'-ACCCA-3' sequence present in stem-loop structure. ERI-1 and a stem-loop binding protein (SLBP) target opposite faces of a unique highly conserved stem-loop RNA scaffold towards the 3' end of Histone mRNA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ERI-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 161-295 mapping within an internal region of ERI-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 161-295 mapping within an internal region of ERI-1 of human origin |
| Entrez Gene | 90459 |
|---|
Application
Western blot analysis of ERI-1 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of ERI-1 expression in non-transfected: (A) and human ERI-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ERI-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
