Application
Western blot analysis of CHMP3 expression in non-transfected: (A) and mouse CHMP3 transfected: (B) 293T whole cell lysates.| Product Name | CHMP3 Antibody (FL-222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-222 representing full length CHMP3 of human origin |
| Modification Notes | The charged multivesicular body proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), as well as required for the formation of these bodies. CHMP3, also known as Vps24, associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of CHMP3 strongly inhibits HIV-1 release. CHMP3 is expressed in brain, skeletal muscle, heart, lung, kidney and liver. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CHMP3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-222 representing full length CHMP3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-222 representing full length CHMP3 of human origin |
| Entrez Gene | 51652 |
|---|
Application
Western blot analysis of CHMP3 expression in non-transfected: (A) and mouse CHMP3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CHMP3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
