Application
Western blot analysis of eIF3 p110 expression in non-transfected 293T: (A), human eIF3 p110 transfected 293T: (B) and JAR (C) whole cell lysates.Application
Western blot analysis of eIF3 p110 expression in non-transfected 293T: (A), mouse eIF3 p110 transfected 293T: (B) and F9 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of eIF3 p110 expression in 293T (A) and MIA PaCa-2 (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of eIF3 p110 expression in JAR (A) and JEG-3 (B) whole cell lysates and mouse placenta tissue extract (C).| Product Name | eIF3 p110 Antibody (H-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 611-761 mapping within an internal region of eIF3 p110 of human origin |
| Modification Notes | Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts (1,2). Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation (1-3). Eukaryotic initiation factor-3 (eIF3) is the largest family of eIFs and consists of at least 10 unique subunits (p170, p116, p110, p66, p48, p47, p44, p40, p36, and p35) in mammals (3,4). eIF3 subunit-9 (eIF3-h, eIF3-p116, p116, eIF3-S9, PRT1) is a 873 amino acid component of the eIF3 multi-subunit complex that is involved in ribosomal 48S complex formation (3,5). Association of the eIF3 complex with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of eIF3 p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 611-761 mapping within an internal region of eIF3 p110 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 611-761 mapping within an internal region of eIF3 p110 of human origin |
| Entrez Gene | 8663 |
|---|
Application
Western blot analysis of eIF3 p110 expression in non-transfected 293T: (A), human eIF3 p110 transfected 293T: (B) and JAR (C) whole cell lysates.
Application
Western blot analysis of eIF3 p110 expression in non-transfected 293T: (A), mouse eIF3 p110 transfected 293T: (B) and F9 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of eIF3 p110 expression in 293T (A) and MIA PaCa-2 (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of eIF3 p110 expression in JAR (A) and JEG-3 (B) whole cell lysates and mouse placenta tissue extract (C).| Application Notes | recommended for detection of eIF3 p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
