Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of PKAα/β/γ cat phosphorylation in KNRK whole cell lysate.Application
Western blot analysis of untreated human PKAα cat transfected: (A) and lambda protein phosphatase treated human PKAα cat transfected: (B) 293T whole cell lysates.Application
Western blot analysis of p-PKAα/β/γ cat phosphorylation in 293T (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of p-PKAα/β/γ cat expression in mouse brain tissue extract.Application
Western blot analysis of PKAα cat phosphorylation in non-transfected: (A) and human PKAα cat transfected: (B) 293T whole cell lysates.| Product Name | p-PKAα/β/γ cat Antibody (Thr 198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 198 of PKAα cat of human origin |
| Modification | Phosphoralated |
| Modification Notes | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic subunits have been identified, designated PKAå cat or Cå, PKA∫ cat/C∫ and PKA© cat/C©, that each represent specific gene products. PKAå cat and PKA∫ cat are closely related (93% amino acid sequence similarity), whereas PKA© cat displays 83% and 79% similarity to PKAå cat and PKA∫ cat, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex. The phosphorylation at threonine 198 is cAMP dependent. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Thr 198 phosphorylated PKAα, β and γ cat of human origin and Thr 198 phosphorylated PKAα and β of mouse and rat originby WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 198 of PKA&alpha cat of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 198 of PKAα cat of human origin |
| Entrez Gene | 5566 |
|---|
Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of PKAα/β/γ cat phosphorylation in KNRK whole cell lysate.
Application
Western blot analysis of untreated human PKAα cat transfected: (A) and lambda protein phosphatase treated human PKAα cat transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of p-PKAα/β/γ cat phosphorylation in 293T (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of p-PKAα/β/γ cat expression in mouse brain tissue extract.
Application
Western blot analysis of PKAα cat phosphorylation in non-transfected: (A) and human PKAα cat transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Thr 198 phosphorylated PKAα, β and γ cat of human origin and Thr 198 phosphorylated PKAα and β of mouse and rat originby WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
