Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of acrogranin expression in non-transfected: (A) and human acrogranin transfected: (B) 293T whole cell lysates.| Product Name | acrogranin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-320 mapping near the N-terminus of acrogranin of human origin |
| Modification Notes | Acrogranin (also designated PC cell-derived growth factor (PCDGF), epithelin/granulin precursor or paragranulin) is a glycosylated protein originally purified from the highly tumorigenic, Insulin-independent mouse teratoma PC cell line. Acrogranin is a cysteine-rich molecule whose expression is essential for tumorigenicity in teratoma cells. Acrogranin is expressed in estrogen receptor-positive (ER(+)) human mammary MDA-MB-468 epithelial cells, human breast cancer MCF7 cells and human estrogen-responsive T-47D cells. Secreted acrogranin acts as an autocrine growth factor for breast carcinoma cells and overexpression may play an important role in human breast cancer. Acrogranin stimulates the growth of PC cells as well as 3T3 fibroblasts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of acrogranin, paragranulin, and granulin 1, 2, 3, 4 and 7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-320 mapping near the N-terminus of acrogranin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-320 mapping near the N-terminus of acrogranin of human origin |
| Entrez Gene | 2896 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of acrogranin expression in non-transfected: (A) and human acrogranin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of acrogranin, paragranulin, and granulin 1, 2, 3, 4 and 7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
