Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear and cytoplasmic staining at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Ran BP-M expression in non-transfected 293T: (A) and human Ran BP-M transfected 293T: (B) whole cell lysates and mouse heart tissue extract (C).| Product Name | Ran BP-M Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 516-605 mapping within an internal region of Ran BP-M of human origin |
| Modification Notes | The small Ras-related protein Ran, also designated TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis, and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). Ran BP-M, also designated Ran-binding protein 9, is involved the nucleation of microtubule networks and may also act as an adapter protein in the coupling of membrane receptors to intracellular signaling pathways. It has been implicated in the activation of androgen and glucocorticoid receptor. Ran BP-M contains phosphorylated Serine residues. It can localize to both the nucleus and the cytoplasm, and a phosphorylated form can be associated with the plasma membrane. Ran BP-M is ubiquitously expressed, with highest expression levels in heart, testis, muscle and placenta. The enzymatic activity of the Ran BP-M protein is associated with breast cancer progression and fragile-X mental retardation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ran BP-M isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF , IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 516-605 mapping within an internal region of Ran BP-M of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 516-605 mapping within an internal region of Ran BP-M of human origin |
| Entrez Gene | 10048 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear and cytoplasmic staining at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Ran BP-M expression in non-transfected 293T: (A) and human Ran BP-M transfected 293T: (B) whole cell lysates and mouse heart tissue extract (C).| Application Notes | recommended for detection of Ran BP-M isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF , IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
