Application
Western blot analysis of NDR1/2 expression in U-937 (A) and HEL 92.1.7 (B) nuclear extracts and human PBL whole cell lysate (C).Application
Western blot analysis of NDR1/2 expression in NIH/3T3 (A), PC-12 (B) and RAW 264.7 (C) whole cell lysates and mouse liver (D) and mouse kidney (E) tissue extracts.Application
Western blot analysis of NDR1/2 expression in HeLa whole cell lysate.Application
Western blot analysis of NDR1/2 expression in Jurkat nuclear extract.| Product Name | NDR1/2 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-100 mapping at the N-terminus of NDR1 of human origin |
| Modification Notes | The nuclear Dbf2-related kinases (NDR1 and NDR2) participate in the regulation of cell division and morphology and may be implicated in tumor progression. NDR1 and NDR2 share 86% amino acid identity, but differ in their expression pattern. NDR1 localizes to the nucleus, while NDR2 exhibits punctate cytoplasmic distribution. Also, NDR1 expression appears highest in spleen, lung and thymus, whereas NDR2 shows highest expression in the gastrointestinal tract. However, both NDR1 and NDR2 are regulated by phosphorylation and by the Ca2+-binding protein S-100B. NDR1 and NDR2 may also play a role in the HIV-1 life cycle. Both proteins are cleaved by the HIV-1 protease (PR), which inhibits their enzymatic activity and alters the subcellular localization of NDR2. The genes encoding human NDR1 and NDR2 map to chromosomes 6p21 and 12p11.23, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NDR1 and NDR2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus of NDR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus of NDR1 of human origin |
| Entrez Gene | 11329 |
|---|
Application
Western blot analysis of NDR1/2 expression in U-937 (A) and HEL 92.1.7 (B) nuclear extracts and human PBL whole cell lysate (C).
Application
Western blot analysis of NDR1/2 expression in NIH/3T3 (A), PC-12 (B) and RAW 264.7 (C) whole cell lysates and mouse liver (D) and mouse kidney (E) tissue extracts.
Application
Western blot analysis of NDR1/2 expression in HeLa whole cell lysate.
Application
Western blot analysis of NDR1/2 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of NDR1 and NDR2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
