Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.Application
Western blot analysis of galectin-1 expression in HL-60 (A) and NIH/3T3 (B) whole cell lysates.Application
Western blot analysis of galectin-1 expression in non-transfected: (A) and mouse galectin-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of galectin-1 expression in HL-60 (A) and SK-MEL-28 (B) whole cell lysates.| Product Name | galectin-1 Antibody (H-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-45 mapping at the N-terminus of galectin-1 of human origin |
| Modification Notes | Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. Specifically, Galectin-1 is an autocrine regulator of cell-proliferation that plays a role in the maintenance of G0 and in the control of G2 traverse. Galectin-1, also known as LGALS1, is the protein product of a single gene linked to human chromosome 22q13.1. The Galectin-1 protein contains 135 amino acids, a single internal EcoRI site and a polyadenylation signal. Galectin-1 can localize to both intracellular and extracellular space. Galectin-1 is expressed in human placenta, human lung, HL-6, HepG2 and CEM cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of galectin-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-45 mapping at the N-terminus of galectin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-45 mapping at the N-terminus of galectin-1 of human origin |
| Entrez Gene | 3956 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of galectin-1 expression in HL-60 (A) and NIH/3T3 (B) whole cell lysates.
Application
Western blot analysis of galectin-1 expression in non-transfected: (A) and mouse galectin-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of galectin-1 expression in HL-60 (A) and SK-MEL-28 (B) whole cell lysates.| Application Notes | recommended for detection of galectin-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
