Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.Application
Western blot analysis of DHFR expression in non-transfected 293T: (A), human DHFR transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of DHFR expression in HeLa whole cell lysate.WB
A, Represent Western blots for DHFR, GTPCH1, and NOX2 in each group.| Product Name | DHFR Antibody (FL-187) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of DHFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-187 representing full length DHFR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-187 representing full length DHFR of human origin |
| Entrez Gene | 1719 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.
Application
Western blot analysis of DHFR expression in non-transfected 293T: (A), human DHFR transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of DHFR expression in HeLa whole cell lysate.
WB
A, Represent Western blots for DHFR, GTPCH1, and NOX2 in each group.| Application Notes | recommended for detection of DHFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
