Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic and nuclear staining of neuronal cells and neuropil.Application
Western blot analysis of Homer expression in non-transfected: (A) and human Homer transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Homer expression in IMR-32 whole cell lysate.Application
Western blot analysis of Homer expression in non-transfected: (A) and human Homer transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane and cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed A-10 cells showing cytoplasmic and membrane localization.Application
Western blot analysis of Homer expression in mouse cerebellum (A) and mouse (B) and rat (C) whole brain extracts.| Product Name | Homer Antibody (H-342) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 13-354 of Homer-1b of human origin |
| Modification Notes | Homer family proteins, also designated Vesl (for VASP/Ena-related gene upregulated during seizure) and LTP, are immediate early gene products that bind to group 1 metabotropic glutamate receptors (mGluRs), proteins involved in triggering intracellular calcium release. Unlike Homer-1a, the prototype member of the Homer family, other Homer family members (Homer-1b and -1c, Homer-2a, -2b and -2c and Homer-3) are constitutively expressed and contain a coiled-coil (CC) domain that mediates self-multimerization. Homer-1a is enriched at excitatory synapses, does not multimerize and appears to block the association of mGluRs to CC-Homer proteins. Homer proteins have also been shown to link mGluRs with the inositol triphosphate receptors (IP3R). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of all Homer isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 13-354 of Homer-1b of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 13-354 of Homer-1b of human origin |
| Entrez Gene | 9456 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic and nuclear staining of neuronal cells and neuropil.
Application
Western blot analysis of Homer expression in non-transfected: (A) and human Homer transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Homer expression in IMR-32 whole cell lysate.
Application
Western blot analysis of Homer expression in non-transfected: (A) and human Homer transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).
Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane and cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed A-10 cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of Homer expression in mouse cerebellum (A) and mouse (B) and rat (C) whole brain extracts.| Application Notes | recommended for detection of all Homer isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
