Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of HIG2 expression in non-transfected: (A) and mouse HIG2 transfected: (B) 293T whole cell lysates.| Product Name | HIG2 Antibody (C-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of HIG2 of human origin |
| Modification Notes | Cell growth and viability is compromised by oxygen deprivation (hypoxia). HIG2 (hypoxia-inducible gene 2 protein), also known as HIG-2 or C7orf68, is a 63 amino acid single-pass membrane protein that can be hypoxia induced by glucose deprivation. Expression of HIG2 is increased in cervical cancer cells but inhibited in renal cell carcinoma. When bound to the extracellular domain of frizzled-10, HIG2 enhances oncogenic Wnt signaling and its own transcription, which suggests HIG2 may function as an autocrine growth factor. HIG2 may be a candidate for development of molecular-targeting therapy and could serve as a prominent diagnostic tumor marker for patients with renal carcinomas. The gene encoding HIG2 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Defects in some of the genes localized to chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HIG2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of HIG2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of HIG2 of human origin |
| Entrez Gene | 29923 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of HIG2 expression in non-transfected: (A) and mouse HIG2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HIG2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
