Application
Western blot analysis of CD16 expression in K-562 (A), U-937 (B) and JEG-3 (C) whole cell lysates.| Product Name | CD16 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 131-210 mapping near the C-terminus of CD16 of human origin |
| Modification Notes | CD16, the low affinity Fc© receptor III (Fc© RIII) for IgG, exists both as a polypeptide-anchored form known as Fcg RIIIA or CD16-A in human natural killer cells and macrophages and as a glycosylphosphatidylinositol-anchored form (Fc© RIIIB or CD16-B) in neutrophils. CD16-A requires association of the © subunit of Fc e RI or the Ω subunit of the TCR-CD3 complex for cell surface expression. CD16-B is polymorphic; the two alleles are designated NA1 and NA2. CD16 is one of only four eukaryotic receptors known to exist natively in both the transmembrane (TM) isoform (CD16-A) and glycosylphosphatidylinositol (GPI) isoform (CD16-B). Patients with paroxysmal nocturnal haemoglobinuria (PNH) have only about 10% of the normal levels of CD16 on their neutrophils, whereas the expression of FcRII is unaffected. Analysis of FcRIII expression in cells of PNH patients, known to be deficient in PI-linked proteins, suggests FcRIII is not PI-linked in monocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD16-A and CD16-B of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-210 mapping near the C-terminus of CD16 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-210 mapping near the C-terminus of CD16 of human origin |
| Entrez Gene | 2214 |
|---|
Application
Western blot analysis of CD16 expression in K-562 (A), U-937 (B) and JEG-3 (C) whole cell lysates.| Application Notes | recommended for detection of CD16-A and CD16-B of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
