Application
Western blot analysis of DEK expression in K-562 nuclear extract.Application
Western blot analysis of DEK expression in 293T (A) and HeLa (B) whole cell lysates.| Product Name | DEK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 76-225 mapping within an internal region of DEK of human origin |
| Modification Notes | The mammalian nuclear phosphoprotein DEK is implicated in multiple cellular processes, including transcriptional regulation, mRNA processing, and chromatin remodeling, and is associated with a number of clinical autoimmune and neoplastic conditions (1). DEK, an abundant chromatin-associated protein, changes the topology of DNA in chromatin in vitro (2,3). Although first identified in a fusion with the CAN/Nup214 nucleoporin protein in a specific subtype of acute myelogenous leukemia, DEK is also an autoantigen in patients with pauciarticular onset juvenile rheumatoid arthritis (4-6). Furthermore, the last 65 amino acids of DEK can partially reverse the mutation-prone phenotype of cells from patients with ataxia-telangiectasia (4). The human DEK gene maps to chromosome 6p23(1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DEK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 76-225 mapping within an internal region of DEK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 76-225 mapping within an internal region of DEK of human origin |
| Entrez Gene | 7913 |
|---|
Application
Western blot analysis of DEK expression in K-562 nuclear extract.
Application
Western blot analysis of DEK expression in 293T (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of DEK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
