Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of subset of cells in Islets of Langerhans.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of ODC expression in non-transfected: (A) and human ODC transfected: (B) 293T whole cell lysates.| Product Name | ODC Antibody (H-71) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 391-461 mapping at the C-terminus of ODC of human origin |
| Modification Notes | Ornithine decarboxylase (ODC) is an enzyme that performs the first step in polyamine biosynthesis by converting ornithine to putrescine and CO2. ODC plays an important role in diverse biological processes, including cell growth, differentiation, transformation and apoptosis. The Sp1, c-Myc and c-Fos genes function as transactivators and ZBP-89 as a transrepressor of the ODC promoter. Overexpression of ODC gene plays important roles in cell proliferation and the development of cancer. High levels of protein binding in the ODC promoter are implicated to the elevated constitutive expression of this gene. Elevated polyamine levels lead to downregulation of ODC activity by enhancing the translation of antizyme mRNA, resulting in subsequent binding of antizyme to ODC monomers to target ODC for proteolysis by the 26S Proteosome. DFMO (DL-a-difluoromethylornithine) is an irreversible inhibitor of ODC, which can induce apoptosis and inhibits cell growth. ODC is also associated with angiogenesis, and ODC-overexpressing cells exhibit suppressed expression of Type XVIII Collagen and endostatin, suggesting that overexpression of ODC facilitates endothelial proliferation by suppressing endostatin expression. The ODC gene maps to human chromosme 2p25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ODC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 391-461 mapping at the C-terminus of ODC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 391-461 mapping at the C-terminus of ODC of human origin |
| Entrez Gene | 4953 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of subset of cells in Islets of Langerhans.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of ODC expression in non-transfected: (A) and human ODC transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ODC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
