Application
Western blot analysis of βB1-crystallin expression in non-transfected: (A) and human βB1-crystallin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of βB1-crystallin expression in non-transfected: (A) and mouse βB1-crystallin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of βB1-crystallin expression in non-transfected: (A) and human βB1-crystallin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of β-crystallin expression in mouse eye tissue extract.Application
Immunofluorescence staining of methanol-fixed Y79 cells showing cytoplasmic localization.| Product Name | β-crystallin Antibody (FL-252) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-252 representing full length βB1-crystallin of human origin |
| Modification Notes | Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into a, b, and g families, and the b- and g-crystallins also comprise a superfamily. Crystallins usually contain seven distinctive protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. b-crystallins constitute the major lens structural proteins. They associate into dimers, tetramers, and higher order aggregates. The b-crystallin subfamily is composed of several gene products, including bA1-, bA2-, bA3-, bA4-, bB1-, bB2- and bB3-crystallin. The bA1- and bA3-crystallin proteins are encoded by a single mRNA. They differ by only 17 amino acids, and bA1-crystallin is generated by use of an alternate translation initiation site. The genes for bA4-, bB1-, bB2- and bB3-crystallin are clustered on human chromosome 22q11, while the genes for bA3/A1- and bA2-crystallin map to human chromosomes 17q11 and 2q34, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of βB1-crystallin and, to a lesser extent, βA1/3, A2, A4, B2 and B3-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-252 representing full length &beta B1-crystallin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-252 representing full length βB1-crystallin of human origin |
| Entrez Gene | 1414 |
|---|
Application
Western blot analysis of βB1-crystallin expression in non-transfected: (A) and human βB1-crystallin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of βB1-crystallin expression in non-transfected: (A) and mouse βB1-crystallin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of βB1-crystallin expression in non-transfected: (A) and human βB1-crystallin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of β-crystallin expression in mouse eye tissue extract.
Application
Immunofluorescence staining of methanol-fixed Y79 cells showing cytoplasmic localization.| Application Notes | recommended for detection of βB1-crystallin and, to a lesser extent, βA1/3, A2, A4, B2 and B3-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
