Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of HSP 90 expression in non-transfected: (A) and human HSP 90 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GRP94 expression in non-transfected 293T: (A), mouse GRP94 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.Application
Western blot analysis of GRP 94 expression in NIH/3T3 whole cell lysate.| Product Name | GRP 94 Antibody (H-212) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 200-411 mapping near the N-terminus of GRP 94 of human origin |
| Modification Notes | Heat shock protein (HSP) molecular chaperones are environmental stress-inducible gene products. The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90å, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90∫, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1. TRAP1 (TNF receptor-associated protein 1), also designated HSP 75 is a mitochondrial matrix component that plays a role in the induction of apoptosis in response to reactive oxygen species. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GRP 94 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 200-411 mapping near the N-terminus of GRP 94 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 200-411 mapping near the N-terminus of GRP 94 of human origin |
| Entrez Gene | 7184 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of HSP 90 expression in non-transfected: (A) and human HSP 90 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GRP94 expression in non-transfected 293T: (A), mouse GRP94 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.
Application
Western blot analysis of GRP 94 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of GRP 94 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
