Application
Western blot analysis of Cdc34B expression in Jurkat (A) and OV-90 (B) whole cell lysates.Application
Western blot analysis of Cdc34B expression in Jurkat (A) and C6 (B) whole cell lysates.| Product Name | Cdc34B Antibody (H-51) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 188-238 mapping at the C-terminus of Cdc34B of human origin |
| Modification Notes | The eukaryotic cell division cycle consists of a number of gene-controlled sequences that involve cyclin dependent kinases (Cdks) and cell division control (Cdc) proteins. Cdc34B, also known as UBE2R2 (Ubiquitin-conjugating enzyme E2 R2) or UBC3B, is a 238 amino acid member of the E2 ubiquitin-conjugating enzyme family. Similar to Cdc34, Cdc34B functions to catalytically attach ubiquitin to various proteins, such as ∫-TrCP (an F-box protein that mediates ∫-catenin degradation), via an ATP-dependent reaction that yields AMP, a diphosphate and a ubiquitin-tagged protein. Cdc34B can be phosphorylated by the protein kinase CK2 (Casein kinase II), thereby allowing Cdc34B to regulate ∫-TrCP substrate recognition and, ultimately, enhance ∫-catenin degradation. Due to its ability to control ∫-TrCP activity, Cdc34B is thought to play a key role in cell cycle progression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cdc34B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 188-238 mapping at the C-terminus of Cdc34B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 188-238 mapping at the C-terminus of Cdc34B of human origin |
Application
Western blot analysis of Cdc34B expression in Jurkat (A) and OV-90 (B) whole cell lysates.
Application
Western blot analysis of Cdc34B expression in Jurkat (A) and C6 (B) whole cell lysates.| Application Notes | recommended for detection of Cdc34B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
