Application
Western blot analysis of α-Syntrophin expression in non-transfected 293T: (A), human α-Syntrophin transfected 293T: (B) and A-673 (C) whole cell lysates.Application
Western blot analysis of α-Syntrophin expression in non-transfected: (A) and human α-Syntrophin transfected: (B) 293T whole cell lysates and rat skeletal muscle tissue extract (C).Application
Western blot analysis of α-Syntrophin expression in non-transfected 293T: (A) and human α-Syntrophin transfected 293T: (B) whole cell lysates and rat skeletal muscle tissue extract (C).| Product Name | α-Syntrophin Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 203-267 mapping within an internal region of α-Syntrophin of human origin |
| Modification Notes | The syntrophins are PDZ-domain-containing proteins that facilitate the recruitment of signaling proteins such as NOS1 to the dystrophin-associated protein complex. The syntrophin family are structurally related proteins that contain multiple protein interaction motifs. Syntrophins associate directly with dystrophin, the product of the Duchenne muscular dystrophy locus, and its homologs. å-Syntrophin has an important role in synapse formation and in the organization of utrophin, acetylcholine receptor and acetylcholinester-ase at the neuromuscular synapse. Specifically, NOS1 binds to å-Syntrophin at muscle sarcolemma. ∫2-Syntrophin is a modular adapter. In muscle cells, ∫2-Syntrophin interacts with members of the dystrophin family, which in-cludes utrophin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of α-Syntrophin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 203-267 mapping within an internal region of &alpha -Syntrophin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 203-267 mapping within an internal region of α-Syntrophin of human origin |
| Entrez Gene | 6640 |
|---|
Application
Western blot analysis of α-Syntrophin expression in non-transfected 293T: (A), human α-Syntrophin transfected 293T: (B) and A-673 (C) whole cell lysates.
Application
Western blot analysis of α-Syntrophin expression in non-transfected: (A) and human α-Syntrophin transfected: (B) 293T whole cell lysates and rat skeletal muscle tissue extract (C).
Application
Western blot analysis of α-Syntrophin expression in non-transfected 293T: (A) and human α-Syntrophin transfected 293T: (B) whole cell lysates and rat skeletal muscle tissue extract (C).| Application Notes | recommended for detection of α-Syntrophin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
