Application
Western blot analysis of ATP9A expression in HeLa (A), PC-3 (B) and F9 (C) whole cell lysates.| Product Name | ATP9A Antibody (N-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an N-terminal cytoplasmic domain of ATP9A of human origin |
| Modification Notes | The family of P-type adenosine triphosphates (ATPases), which are phosphorylated in their intermediate state, are involved in the active transport of charged substrates across biological membranes. Members of this family are ubiquitous integral membrane proteins and can be divided into 5 major groups consisting of several subfamilies each. The P-type ATPase Type IV family members are characterized as phospholipid pumps and are then divided into 6 classes determined by sequence similarity. ATP9A (ATPase class II type 9A) is a 1,047 multi-pass transmembrane protein that uses ATP to maintain ion gradients across the cell membrane and may possess some aminophospholipid translocase activity. ATP9A is strongly expressed in all tissues, with lower expression found in spleen. There are two named isoforms of ATP9A characterized as long and short forms which are a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ATP9A of mouse and human origin by WB, IP, IF and ELISA non cross-reactive with ATP9B also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an N-terminal cytoplasmic domain of ATP9A of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an N-terminal cytoplasmic domain of ATP9A of human origin |
| Entrez Gene | 10079 |
|---|
Application
Western blot analysis of ATP9A expression in HeLa (A), PC-3 (B) and F9 (C) whole cell lysates.| Application Notes | recommended for detection of ATP9A of mouse and human origin by WB, IP, IF and ELISA non cross-reactive with ATP9B also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
