Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.Application
Western blot analysis of ARFRP1 expression in non-transfected: (A) and mouse ARFRP1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of ARFRP1 expression in non-transfected: (A) and mouse ARFRP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARFRP1 expression in non-transfected: (A) and mouse ARFRP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARFRP1 expression in Hep G2 whole cell lysate.| Product Name | ARFRP1 Antibody (E-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of ARFRP1 of human origin |
| Modification Notes | The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells and they play a central role in the maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D (PC-PLD). ARFRP1 (ADP-ribosylation factor related protein 1), also known as ARP or ARL18, is a 201 amino acid membrane-associated GTPase that localizes to the plasma membrane and the Golgi apparatus and is related to the ARF family of regulatory proteins. Expressed in a variety of tissues, ARFRP1 interacts with SYS1 and is thought to be involved in plasma membrane-related signaling events. ARFRP1 exists as multiple alternatively spliced isoforms and is encoded by a gene which maps to a gene cluster on chromosome 20 that is commonly overexpressed in tumors, suggesting a role for ARFRP1 in carcinogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ARFRP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of ARFRP1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of ARFRP1 of human origin |
| Entrez Gene | 10139 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.
Application
Western blot analysis of ARFRP1 expression in non-transfected: (A) and mouse ARFRP1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of ARFRP1 expression in non-transfected: (A) and mouse ARFRP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARFRP1 expression in non-transfected: (A) and mouse ARFRP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARFRP1 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of ARFRP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
