Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Western blot analysis of Tankyrase-1/2 expression in untreated (A) and XAV939 treated (B) SW480 whole cell lysates. Note upregulation of Tankyrase-1/2 expression in lane B.Application
Western blot analysis of Tankyrase-1/2 expression in A549 (A) and Jurkat (B) whole cell lysates and rat testis tissue extract (C).| Product Name | Tankyrase-1/2 Antibody (H-350) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 745-1094 of Tankyrase -1 of human origin. |
| Modification Notes | Tankyrase-1 (TRF1-interacting ankyrin-related ADP-ribose polymerase 1) and the closely related homolog Tankyrase-2 arepoly(ADP-ribose) polymerases (PARPs) that co-localize and use an ankyrin-repeat domain to bind diverse proteins, including TRF-1 (telomere-repeat-binding factor 1), IRAP (insulin-responsive aminopeptidase) and TAB182. Tankyrase-1 (also known as TNKS and TNKS1) and Tankyrase-2 (also known as TNKS2, TNKL and TANK2) interact with the same set of proteins and probably mediate overlapping functions, both at telomeres and in vesicular compartments. Overexpression of Tankyrase-1 in the nucleus promotes telomere elongation, suggesting that Tankyrase 1 may regulate access of telomerase to the telomeric complex. Overexpression of Tankyrase-2 in the nucleus releases endogenous TRF1 from telomeres, establishing Tankyrase-2 as a PARP with itself and TRF1 as acceptors of ADP-ribosylation, and suggesting the possibility of a role for Tankyrase-2 at telomeres. The ankyrin (ANK) domain of Tankyrase-2 comprises five subdomains that provide redundant binding sites for IRAP. Tankyrase-2 lacks the N-terminal Histidine/Proline/Serine-rich region of Tankyrase-1, but contains a corresponding ankyrin repeat region, sterile å motif module and poly(ADP-ribose) polymerase homology domain. The gene encoding Tankyrase-2 is widely expressed, with mRNA transcripts particularly abundant in skeletal muscle and placenta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Tankyrase-1 and -2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 745-1094 of Tankyrase -1 of human origin. |
|---|---|
| Tissue Specificity | raised against amino acids 745-1094 of Tankyrase -1 of human origin. |
| Entrez Gene | 8658 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Western blot analysis of Tankyrase-1/2 expression in untreated (A) and XAV939 treated (B) SW480 whole cell lysates. Note upregulation of Tankyrase-1/2 expression in lane B.
Application
Western blot analysis of Tankyrase-1/2 expression in A549 (A) and Jurkat (B) whole cell lysates and rat testis tissue extract (C).| Application Notes | recommended for detection of Tankyrase-1 and -2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
