Application
Western blot analysis of IRS-3 expression in rat pancreas tissue extract.| Product Name | IRS-3 Antibody (R-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 305-494 mapping at the C-terminus of IRS-3 of rat origin |
| Modification Notes | Insulin receptor substrate (IRS) proteins play important roles in insulin action and pancreatic beta-cell function. IRS-3, identified only in rodents, shows robust and prolonged tyrosine phosphorylation upon insulin treatment of cells and may play a role in delayed and prolonged insulin actions. IRS-3 interacts with phosphatidylinositol-3-kinase in adipocytes and hepatoma cells and contains pleckstrin and phosphotyrosine binding domains which are highly homologous to domains in IRS-1 and IRS-2. IRS-3 exhibits an expression pattern which differs from other IRS proteins. During both the embryonic development and adult life of the mouse, p53 inhibits the IRS-3 promoter, while tumor-derived p53 mutants de-repress the same promoter. GFP-IRS-3 fusion protein and endogenous rat IRS-3 localize in both the plasma membrane and the nucleus, indicating that intracellular localization of IRS-3 is determined by a different mechanism from other IRS proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IRS-3 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 305-494 mapping at the C-terminus of IRS-3 of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 305-494 mapping at the C-terminus of IRS-3 of rat origin |
| Entrez Gene | 16369 |
|---|
Application
Western blot analysis of IRS-3 expression in rat pancreas tissue extract.| Application Notes | recommended for detection of IRS-3 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
