Application
Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.Application
Western blot analysis of IL-8RB expression in whole cell lysates prepared from CHO-K1 cells transfected with IL-8RB (A) and non-transfected CHO-K1 cells (B).Application
Intracellular FCM analysis of fixed and permeabilized non-transfected (green line histogram) and IL-8RB transfected (solid orange histogram) CHO-K1 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .| Product Name | IL-8RB Antibody (K-19) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of IL-8RB of mouse origin |
| Modification Notes | IL-8 has been shown to function as a potent neutrophil chemostatic and activating peptide and is an important mediator of inflammatory diseases. Two distinct human IL-8 receptors, designated IL-8RA and IL-8RB, have been characterized. Both are expressed at a high level on neutrophils, and to a lesser extent on monocytes and myeloid cell lines. In addition, the IL-8RA subunit is expressed in T cells such as the Jurkat cell line. Both IL-8Rs are members of the seven transmembrane domain rhodopsin superfamily of receptors and as such, couple G proteins for signal transduction. The two receptors share 77% amino acid identity. IL-8RA exhibits high affinity binding for IL-8 and low affinity MGSA binding, whereas IL-8RB has high affinity binding for both IL-8 and MGSA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of IL-8RB of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, FCM and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of IL-8RB of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of IL-8RB of mouse origin |
| Entrez Gene | 3579 |
|---|
Application
Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.
Application
Western blot analysis of IL-8RB expression in whole cell lysates prepared from CHO-K1 cells transfected with IL-8RB (A) and non-transfected CHO-K1 cells (B).
Application
Intracellular FCM analysis of fixed and permeabilized non-transfected (green line histogram) and IL-8RB transfected (solid orange histogram) CHO-K1 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .| Application Notes | recommended for detection of IL-8RB of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, FCM and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
