Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Antibodies tested include p-PKR (Thr 451): (A,B,C) and PKR (M-515): (D,E,F).Application
Antibodies tested include p-PKR (Thr 446)-R: (A,B,C) and PKR (M-515): (D,E,F).Application
Western blot analysis of PKR expression in non-transfected: (A) and mouse PKR transfected: (B) 293T whole cell lysates.Application
Antibodies tested include PKR (M-515): (A) and PKR (B-10): (B).| Product Name | PKR Antibody (M-515) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-515 representing full length PKR of mouse origin |
| Modification Notes | An interferon-inducible, RNA-dependent protein serine/threonine kinase,PKR, has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the a subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2a on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA mediated autophosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PKR of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-515 representing full length PKR of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-515 representing full length PKR of mouse origin |
| Entrez Gene | 19106 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Antibodies tested include p-PKR (Thr 451): (A,B,C) and PKR (M-515): (D,E,F).
Application
Antibodies tested include p-PKR (Thr 446)-R: (A,B,C) and PKR (M-515): (D,E,F).
Application
Western blot analysis of PKR expression in non-transfected: (A) and mouse PKR transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include PKR (M-515): (A) and PKR (B-10): (B).| Application Notes | recommended for detection of PKR of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
