| Product Name | DOHH Antibody (FL-302) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-302 representing full length DOHH of human origin |
| Modification Notes | DOHH (deoxyhypusine hydroxylase/monooxygenase), also known as HLRC1 (HEAT-like (PBS lyase) repeat containing 1), is a metalloenzyme involved in hypusine synthesis. It contains eight tandem HEAT-repeats, four at the N-terminus and four at the C-terminus. DOHH is an important player in mediating the posttranslational modifications of eIF5a to form hypusine. The first step of this reaction is catalyzed by DHS (deoxyhypusine synthase), which is responsible for transferring the aminobutyl moiety of spermidine to a lysine residue of eIF5a to form a deoxyhypusine-containing eIF5a intermediate. DOHH catalyzes the second step, hydroxylating the intermediate to form the hypusine residue thereby activating eIF5a. DHS, DOHH and eIF5a are evolutionarily conserved proteins that are essential for cell proliferation. Inhibition of DOHH can result in cell cycle arrest at the G1/S boundary. This suggests a potential use of DOHH inhibitors in antitumor therapy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DOHH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-302 representing full length DOHH of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-302 representing full length DOHH of human origin |
| Entrez Gene | 83475 |
|---|
| Application Notes | recommended for detection of DOHH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
