Application
Western blot analysis of Rex-1 expression in mouse kidney tissue extract.Application
Western blot analysis of Rex-1 expression in mouse kidney tissue extract (A) and F9 whole cell lysate (B).| Product Name | Rex-1 Antibody (M-149) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 27-175 mapping within an internal region of Rex-1 of mouse origin |
| Modification Notes | Rex-1 (for reduced expression), also designated zinc finger protein 42 (ZFP42), is an acidic zinc finger protein. Rex-1 contains four repeats of the zinc finger nucleic acid-binding motif and a potential acidic activator domain, suggesting that it is a regulatory protein. Rex-1 localizes to the nucleus and is highly expressed in embryonic stem (ES) and undifferentiated murine F9 teratocarcinoma cells. At the transcriptional level, expression of Rex-1 is reduced when F9 cells are induced to differentiate by the addition of retinoic acid (RA), and Rex-1 repression is enhanced by E1A. The Oct-3/4 transcription factor can either activate or repress the Rex-1 promoter, depending on the cellular environment, while Oct-6 can lower the expression of Rex-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rex-1 (zinc finger protein 42) of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 27-175 mapping within an internal region of Rex-1 of mouse origin |
|---|---|
| Tissue Specificity | raised against amino acids 27-175 mapping within an internal region of Rex-1 of mouse origin |
Application
Western blot analysis of Rex-1 expression in mouse kidney tissue extract.
Application
Western blot analysis of Rex-1 expression in mouse kidney tissue extract (A) and F9 whole cell lysate (B).| Application Notes | recommended for detection of Rex-1 (zinc finger protein 42) of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
